|
KMID : 0377220000250010039
|
|
Medical Journal of Chosun Univercity
2000 Volume.25 No. 1 p.39 ~ p.44
|
|
|
Usefulness of Competitive Enzyme Immunoassay for Detection of Treponemal Antibody in Serum
|
|
Choi Ha-Young
Park Geon
Jang Sook-Jin
Park Young-Jin
Moon Dae-Soo
|
|
|
Abstract
|
|
|
Background : Laboratory methods for detection of syphilis are Venereal Disease Research Laboratory (VDRL) and rapid plasma reagin (RPR) test to non-treponemal tests, and fluorescent treponemal antibody absorption (FTA-ABS) and microhemagglutination assay for antibodies to Treponema pallidum (MHA-TP) to treponemal tests. However, RPR card test had many false positive results and FTA-ABS IgM method required high degrees of technical ability and experience. Recently, enzyme immunoassay (EIA) kits for IgM and IgG antibodies to T. pallidum in syphilis have been developed. So, we evaluated that usefulness of competitive EIA for detection of T. pallidum antibody in serum.
Methods : RPR positive 38 specimens and negative 6 specimens were compared by T. pallidum hemagglutination (TPHA), two commercial competitive EIA kits for detection of syphilis and FTA-ABS IgM. Those data were compared with concordant and discordant rate of each RPR, TPHA, EIA and FTA-ABS IgM.
Results : 28 of 34 RPR positive specimens were TPHA(+) and EIA (+), and 6 of 6 RPR negative specimens were TPHA (-) and EIA (-). The data of two EIAs were same results with TPHA. 3 of 28 EIA positive specimens were FTA-ABS IgM (+), and 16 of 16 EIA negative specimens were FTA-ABS IgM (-).
Conclusion: Two commercial competitive EIA kits for detection of syphilis were evaluated. The EIA method could be replaced with RPR and TPHA, and this method was possible to combination for detection of syphilis.
|
|
|
KEYWORD
|
|
|
Syphilis, Rapid plasma reagin, RPR, Enzyme immunoassay, EIA, Fluorescent treponemal antibody absorption, FTA-ABS
|
|
|
FullTexts / Linksout information
|
|
|
|
|
|
Listed journal information
|
|
|
|
|